STRUCTURAL AND DEVELOPMENTAL ANALYSIS OF A GENE CLONED FROM THE EARLY ECDYSTERONE-INDUCIBLE PUFF SITE, I-18C, IN CHIRONOMUS-TENTANS

被引：7

作者：

DORSCHHASLER, K ^{[1
]}

LUTZ, B ^{[1
]}

SPINDLER, KD ^{[1
]}

LEZZI, M ^{[1
]}

机构：

[1] UNIV DUSSELDORF,INST ZOOL,W-4000 DUSSELDORF 1,GERMANY

来源：

GENE | 1990年 / 96卷 / 02期

关键词：

alternative splicing; developmental regulation; Northern blots; primer extension; Recombinant DNA; S1; nuclease;

D O I：

10.1016/0378-1119(90)90258-S

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A gene (I-18C) cloned from the early ecdysterone (EDS)-inducible puff site, I-18C, in Chironomus tentans salivary glands, codes by differential processing for at least five transcripts which can be grouped into spliced and unspliced transcripts. The spliced group comprises several RNAs of approx. 4.6 kb, and the unspliced group comprises the 1.8-kb and 6.5-kb RNAs. All these transcripts have a similar or the same transcription start point. The steady-state level of the spliced 4.6-kb RNAs reveals some parallels with EDS concentrations in animals during the various developmental stages analyzed, while the levels of the unspliced 1.8-kb and 6.5-kb RNAs do not correlate with the EDS concentrations. © 1990.

引用

页码：233 / 239

页数：7

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