DISTINCT DISTRIBUTIONS OF 2 BOMBESIN RECEPTOR SUBTYPES IN THE RAT CENTRAL-NERVOUS-SYSTEM

被引：66

作者：

LADENHEIM, EE ^{[1
]}

JENSEN, RT ^{[1
]}

MANTEY, SA ^{[1
]}

MORAN, TH ^{[1
]}

机构：

[1] NIDDKD, BETHESDA, MD 20892 USA

来源：

BRAIN RESEARCH | 1992年 / 593卷 / 02期

关键词：

BOMBESIN; GASTRIN-RELEASING PEPTIDE; NEUROMEDIN-B; RECEPTOR AUTORADIOGRAPHY; CENTRAL NERVOUS SYSTEM;

D O I：

10.1016/0006-8993(92)91305-X

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We have previously demonstrated the presence of two distinct bombesin receptor subtypes in the rat CNS and distinguished them as bombesin/gastrin-releasing peptide (BBS/GRP) and neuromedin B (NMB)-preferring binding sites. In the present study, we conduct a complete evaluation of the distribution of these binding sites throughout the rat brain using in vitro receptor autoradiography. The BBS/GRP-preferring binding sites were characterized as those that bound I-125-(Tyr4)BBS but not I-125-(D-Tyro)NMB. At these sites I-125-(Tyr4)BBS binding was inhibited in the presence of 100 nM BBS but not by the same concentration of NMB. In contrast, NMB-preferring sites bound both radioligands and binding at these sites was inhibited in the presence of 100 nM NMB. Our results indicate that the distributions of BBS/GRP and NMB-preferring binding sites are widespread and distinct at all levels of the rat brain suggesting these peptides mediate separate functions in the rat central nervous system.

引用

页码：168 / 178

页数：11