STIMULATION BY RECOMBINANT HUMAN GROWTH-HORMONE OF GROWTH AND DEVELOPMENT OF REMAINING BOWEL AFTER SUBTOTAL ILEOJEJUNECTOMY IN RATS

被引:32
作者
BENHAMOU, PH [1 ]
CANARELLI, JP [1 ]
LEROY, C [1 ]
DEBOISSIEU, D [1 ]
DUPONT, C [1 ]
机构
[1] CHRU AMIENS, AMIENS, FRANCE
关键词
HUMAN RECOMBINANT GROWTH HORMONE; SMALL BOWEL RESECTION; SHORT BOWEL;
D O I
10.1097/00005176-199405000-00007
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
The impact of human recombinant growth hormone (GH) after massive small bowel resection was studied in 38 weaning female Wistar rats (65 +/- 5 days old; 193 +/- 26 g). Animals underwent a 80% small bowel resection, leaving in place similar lengths of jejunum and ileum. Animals were assigned to four groups: group A (n = 9), small bowel resection only; group B (n = 10), resection and treatment with 0.2 GH units (GHU) s.c. every other day; group C (n = 9), resection and 0.4 GHU; and group D (n = 10), laparotomy without intestinal resection. Twenty-eight days later, weight gain (percentage of initial weight) was 1 +/- 3 in group A, 12 +/- 8 in group B, 12 +/- 9 in group C, and 16 +/- 7 in group D; p < 0.001, groups B-D. Time to recover initial weight was 26.2 +/- 3.3 days in group A; 11.7 +/- 5.4 days in group B (p < 0.001); and 16 +/- 6.1 days in group C (p < 0.001 vs. A). The size of the intestinal remnant after the rats were killed was 1.3 +/- 0.6 cm (13 +/- 10% of initial length) in A; 5.15 +/- 2.4 cm (37 +/- 18%) in B (p < 0.01); 4.2 +/- 2.3 cm (33 +/- 20%) in C (p < 0.01 vs. A); and 4.4 +/- 3.5 cm (5.8 +/- 4.9%) in D (p < 0.001 vs A). Villus height and diameter, average number of mitosis per field, and muscular layer and wall thickness were greater in groups A, B, and C than in group D (p < 0.001). Villus height and muscular thickness of the jejunal part were increased in group C compared with A (p < 0.05). In conclusion, GH improves the postoperative intestinal adaptation process in terms of both weight gain and small bowel lengthening.
引用
收藏
页码:446 / 452
页数:7
相关论文
共 30 条
  • [11] REGULATION OF GASTROINTESTINAL MUCOSAL GROWTH
    JOHNSON, LR
    [J]. PHYSIOLOGICAL REVIEWS, 1988, 68 (02) : 456 - 502
  • [12] GROWTH-HORMONE RECEPTOR EXPRESSION IN THE RAT GASTROINTESTINAL-TRACT
    LOBIE, PE
    BREIPOHL, W
    WATERS, MJ
    [J]. ENDOCRINOLOGY, 1990, 126 (01) : 299 - 306
  • [13] INFLUENCE OF OVINE GROWTH-HORMONE ON WATER AND NACL ABSORPTION BY THE RAT PROXIMAL JEJUNUM AND DISTAL ILEUM
    MAINOYA, JR
    [J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-PHYSIOLOGY, 1982, 71 (03): : 477 - 479
  • [14] MANSON JM, 1986, SURGERY, V100, P188
  • [15] MCDERMOTT FT, 1976, GASTROENTEROLOGY, V70, P707
  • [16] ROLE OF ORAL INTAKE ON INTESTINAL ADAPTATION AFTER SMALL BOWEL RESECTION IN GROWING RATS
    MORIN, CL
    LING, V
    VANCAILLIE, M
    [J]. PEDIATRIC RESEARCH, 1978, 12 (04) : 268 - 271
  • [17] SHORT BOWEL SYNDROME
    NIGHTINGALE, JMD
    WALKER, ER
    BURNHAM, WR
    FARTHING, MJG
    LENNARDJONES, JE
    [J]. DIGESTION, 1990, 45 : 77 - 83
  • [18] OBERTOP H, 1977, GASTROENTEROLOGY, V72, P267
  • [19] OSCARSON JEA, 1977, GASTROENTEROLOGY, V72, P890
  • [20] PORUS RL, 1965, GASTROENTEROLOGY, V48, P753