Biotransformation of saponins to astragaloside IV from Radix Astragali by immobilized Aspergillus niger

被引：10

作者：

Chen, Cai-Yun ^{[2
]}

Fu, Yu-Jie ^{[1
,3
]}

Zu, Yuan -Gang ^{[1
,3
]}

Wang, Wei ^{[1
,3
]}

Mu, Fan -Song ^{[1
,3
]}

Luo, Meng ^{[1
,3
]}

Li, Chun-Ying ^{[1
,3
]}

Gu, Cheng-Bo ^{[1
,3
]}

Zhao, Chun-Jian ^{[1
,3
]}

机构：

[1] Northeast Forestry Univ, State Engn Lab Bioresource Ecoutilizat, Harbin 150040, Heilongjiang, Peoples R China

[2] Binzhou Med Coll, Coll Pharm, Yantai 264003, Peoples R China

[3] Northeast Forestry Univ, Engn Res Ctr Forest Biopreparat, Minist Educ, Harbin 150040, Heilongjiang, Peoples R China

来源：

BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY | 2013年 / 2卷 / 03期

关键词：

Astragaloside IV; Fermentation; Immobilized cells; Production kinetics; Radix Astragali;

D O I：

10.1016/j.bcab.2013.03.007

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Eight different strains of yeasts, Aspergillus niger, Aspergillus oryzae and White-rot fungus were tested for biotransformation of saponins to astragaloside IV in potato medium with Radix Astragali. Among these strains, immobilized cells of A. niger M85 exhibited the best performance on the biotransformation of saponins to astragaloside IV from Radix Astragali. After the biotransformation with immobilized A. niger M85, the contents of astragaloside IV and total astragalosides in Radix Astragali increased 10.7-fold (2.326 mg/g) and 8.6-fold (2.56 mg/g) to the solvent extraction sample, respectively. Optimization was done at incubation period 5 days, the amount of Radix Astragali powder 2 g, medium volume 50 mL/g, incubation temperature 30 degrees C and initial pH 6.0. The immobilized A. niger M85 can be reused seven times and retained 71% of its residual activity. The A. niger M85 was found to be a highly efficient producer of astragaloside IV. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：196 / 203

页数：8

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