PURIFICATION OF ALPHA-2-MACROGLOBULIN WITH TRYPSIN-LIKE ACTIVITY FROM PLEURAL FLUIDS

被引：4

作者：

BIETH, J ^{[1
]}

KLUMPP, T ^{[1
]}

机构：

[1] UNIV LOUIS PASTEUR, UER SCI PHARM, CHIM BIOL LAB, F-67083 STRASBOURG, FRANCE

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1976年 / 439卷 / 02期

关键词：

D O I：

10.1016/0005-2795(76)90072-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An .alpha.2-macroglobulin with trypsin-like activity was purified from pleural fluids of patients suffering from chronic pancreatitis. The isolation procedure includes ammonium sulphate precipitation, gel-filtration on Sephadex G-200 and DEAE-cellulose chromatography. It gives 46-fold purification of .alpha.2-macroglobulin with a 13% recovery. Based on titration experiments with pancreatic inhibitor, the protein from 3 different patients contained 0.28, 0.46 and 0.80 mol of trypsin-like protease mol of .alpha.2-macroglobulin.

引用

页码：363 / 367

页数：5

共 16 条

[1] INTERACTION OF ALPHA2-MACROGLOBULIN WITH PROTEINASES - CHARACTERISTICS AND SPECIFICITY OF REACTION, AND A HYPOTHESIS CONCERNING ITS MOLECULAR MECHANISM [J].

BARRETT, AJ ;

STARKEY, PM .

BIOCHEMICAL JOURNAL, 1973, 133 (04) :709-&

[2] DETECTION AND DETERMINATION OF ALPHA2-MACROGLOBULIN TRYPSIN ACTIVITY IN PLEURAL FLUIDS AND ASCITES [J].

BIETH, J ;

METAIS, P ;

WARTER, J .

ENZYME, 1971, 12 (01) :13-&

[3] ELASTASE INHIBITORS AS IMPURITIES IN COMMERCIAL PREPARATIONS OF KUNITZ SOYBEAN TRYPSIN-INHIBITOR [J].

BIETH, J ;

FRECHIN, JC .

BIOCHIMICA ET BIOPHYSICA ACTA, 1974, 364 (01) :97-102

[4] ENZYMATIC AND IMMUNOLOGIC INHIBITORS OF PROTEASES IN CSF [J].

BIETH, J ;

MIESCH, F ;

METAIS, P .

CLINICA CHIMICA ACTA, 1969, 24 (02) :203-&

[5] FRACTIONATION AND PROPERTIES OF PROTEASE INHIBITORS OF EFFUSIONS [J].

BIETH, J ;

BARTH, JG ;

METAIS, P .

CLINICA CHIMICA ACTA, 1970, 30 (03) :621-&

[6]

BIETH J, 1966, ANN BIOL CLIN-PARIS, V24, P787

[7] INFLUENCE OF ALPHA2-MACROGLOBULIN ON ELASTOLYTIC AND ESTEROLYTIC ACTIVITY OF ELASTASE [J].

BIETH, J ;

PICHOIR, M ;

METAIS, P .

FEBS LETTERS, 1970, 8 (06) :319-&

[8] P-NITROPHENYL-P'-GUANIDINOBENZOATE HCL - A NEW ACTIVE SITE TITRANT FOR TRYPSIN [J].

CHASE, T ;

SHAW, E .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1967, 29 (04) :508-&

[9] DISC ELECTROPHORESIS .2. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS [J].

DAVIS, BJ .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1964, 121 (A2) :404-&

[10]

GANROT PO, 1967, ARK KEMI, V26, P583

← 1 2 →