THE INVITRO DEVELOPMENT OF CYTOTOXICITY IN RESPONSE TO GRANULOCYTE MACROPHAGE-COLONY-STIMULATING FACTOR OR INTERFERON-GAMMA IN THE PERIPHERAL-BLOOD MONOCYTES OF PATIENTS WITH SOLID TUMORS - MODULATION BY ARACHIDONIC-ACID METABOLIC-INHIBITORS

The capacity of granulocyte/macrophage-colony-stimulating factor (GM-CSF) and interferon γ (IFN γ) to elicit monocyte cytotoxicity in vitro in the peripheral blood monocytes of patients with solid tumors was investigated. The cytotoxicity elicited by IFN γ was significantly reduced in cancer patient monocytes compared to normal monocytes. The cytotoxicity elicited by GM-CSF, however, was comparable between cancer patient monocytes and normal monocytes, but was lower than that induced by IFN γ. Indomethacin, a cyclooxygenase inhibitor, significantly augmented IFN γ-elicited cytotoxicity in cancer patient monocytes, but not in normal monocytes. In contrast, indomethacin augmented GM-CSF-elicited cytotoxicity in both cancer patient monocytes and normal monocytes. Nordihydroguaiaretic acid (NDGA), a lipoxygenase inhibitor, was found to suppress cytotoxicity in response to IFN γ and GM-CSF in both cancer patient monocytes and normal monocytes. The addition of leukotrienes to NDGA-treated cultures restored the development of cytotoxicity. Thus there are differences in the in vitro response of cancer patient monocytes and normal monocytes to distinct biological activators. Furthermore, these responses can be manipulated by agents that modulate arachidonic acid metabolism. © 1990 Springer-Verlag.