GENES ENCODING THE TI BETA-SUBUNIT OF THE ANTIGEN MHC RECEPTOR UNDERGO REARRANGEMENT DURING INTRATHYMIC ONTOGENY PRIOR TO SURFACE T3-TI EXPRESSION

被引：148

作者：

ROYER, HD

ACUTO, O

FABBI, M

TIZARD, R

RAMACHANDRAN, K

SMART, JE

REINHERZ, EL

机构：

[1] HARVARD UNIV, SCH MED,DEPT MED,DANA FARBER CANC INST, DIV TUMOR IMMUNOL, 44 BINNEY ST, BOSTON, MA 02115 USA

[2] BIOGEN RES CORP, CAMBRIDGE, MA 02142 USA

[3] HARVARD UNIV, SCH MED, DEPT PATHOL, BOSTON, MA 02115 USA

来源：

CELL | 1984年 / 39卷 / 02期

关键词：

D O I：

10.1016/0092-8674(84)90003-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To obtain further information about the ontogeny of the T-cell antigen/MHC [major histocompatibility complex] receptor, a Ti .beta. subunit complementary DNA probe and heteroantisera specific for the Ti .alpha. and Ti .beta. subunits were utilized to characterize human T-lineage cells. Analysis of thymic tumors and normal thymocytes at the DNA and protein levels demonstrates that Ti .beta. gene rearrangement is evident in stage II (T11+T6+T3-) and stage III (T11+T6-T3+), but not stage I (T11+T6-T3-) thymocytes. Surface expression of Ti .alpha. and Ti .beta. molecules is exclusively restricted to stage III thymocytes. Human T-lineage ontogeny is characterized by an orderly series of differentiation steps wherein Ti .beta. gene rearrangement precedes surface expression of the T3-Ti molecular complex.

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页码：261 / 266

页数：6

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