PROMISCUITY OF GABA-A-RECEPTOR BETA-3 SUBUNITS AS DEMONSTRATED BY THEIR PRESENCE IN ALPHA-1, ALPHA-2 AND ALPHA-3 SUBUNIT-CONTAINING RECEPTOR SUBPOPULATIONS

被引：27

作者：

POLLARD, S ^{[1
]}

DUGGAN, MJ ^{[1
]}

STEPHENSON, FA ^{[1
]}

机构：

[1] UNIV LONDON,SCH PHARM,DEPT PHARMACEUT CHEM,29-39BRUNSWICK SQ,LONDON WC1N 1AX,ENGLAND

来源：

FEBS LETTERS | 1991年 / 295卷 / 1-3期

基金：

英国医学研究理事会;

关键词：

GABA-A; RECEPTOR; BENZODIAZEPINE; ANTIBODY; IMMUNOAFFINITY; PURIFICATION;

D O I：

10.1016/0014-5793(91)81390-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Polyclonal antibodies were raised in rabbits against the GABA(A)-receptor beta-3 subunit peptide sequence, KQSMPREGHGRHMDR-NH2, coupled to keyhole limpet haemocyanin. These anti-beta-3 379-393 antibodies immunoprecipitated in a dose-dependent manner specific benzodiazepine agonist binding sites from Na+ deoxycholate extracts of bovine cerebral cortex. In immunoblots, anti-beta-3 379-393 antibodies recognised two species with M(r) 59900 and M(r) 57200 in all preparations tested, which included crude detergent-solubilised, benzodiazepine affinity chromatography-purified receptor, anti-alpha-1 324-341 antibody, anti-Cys alpha-2 414-424 antibody and anti-Cys alpha-3 454-467 antibody immunoaffinity-purified GABA(A)-receptor subpopulations. These results provide evidence for the ubiquity and promiscuity of the GABA(A)-receptor beta-3 subunit.

引用

页码：81 / 83

页数：3

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