BENZOYL-COENZYME-A 3-MONOOXYGENASE, A FLAVIN-DEPENDENT HYDROXYLASE - PURIFICATION, SOME PROPERTIES AND ITS ROLE IN AEROBIC BENZOATE OXIDATION VIA GENTISATE IN A DENITRIFYING BACTERIUM

被引:21
作者
NIEMETZ, R
ALTENSCHMIDT, U
BRUCKER, S
FUCHS, G
机构
[1] UNIV ULM,D-89069 ULM,GERMANY
[2] UNIV FREIBURG,INST BIOL 2,FREIBURG,GERMANY
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1995年 / 227卷 / 1-2期
关键词
BENZOYL-COENZYME-A; 3-MONOOXYGENASE; FLAVIN; HYDROXYLASE; AEROBIC BENZOATE PATHWAY; PSEUDOMONAS;
D O I
10.1111/j.1432-1033.1995.tb20372.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A new variant of aerobic benzoate degradation has been found in a denitrifying bacterium in which benzoyl-CoA is the first intermediate [Altenschmidt, U., Oswald, B., Steiner, E., Herrmann, H. and Fuchs, G. (1993) New aerobic benzoate oxidation pathway via benzoyl-coenzyme A and 3-hydroxybenzoyl-coenzyme A in a denitrifying Pseudomonas sp, J. Bacteriol. 175, 4851-4858)]. The initial reaction is catalyzed by benzoate-CoA ligase (AMP-forming), converting benzoate into benzoyl-CoA. The next step is 3-hydroxylation of benzoyl-CoA to 3-hydroxybenzoyl-CoA catalyzed by a flavin-nucleotide-dependent monooxygenase, benzoyl-CoA 3-monooxygenase. This novel enzyme has been purified and studied. It is specific for NADPH and requires the presence of a flavin nucleotide for activity; both FAD or FMN function similarly well as cofactor. Only benzoyl-CoA, but not benzoate, is hydroxylated. The protein is a monomer of M(r) 65 000 and is induced when cells are grown aerobically with benzoate. 3-Hydroxybenzoyl-CoA is further hydroxylated para to the hydroxyl group affording 2,5-dihydroxybenzoate (gentisate). This reaction requires another monooxygenase, 3-hydroxybenzoyl-CoA 6-monooxygenase, which is unspecific with respect to the pyridine nucleotide. Cells contain a second 6-monooxygenase activity which acts on free 3-hydroxybenzoate. Based on these and other data, the outlines of the new aerobic benzoate pathway have been deduced. The proposed intermediates are benzoyl-CoA, 3-hydroxybenzoyl-CoA, gentisate, maleylpyruvate, fumarylpyruvate and fumarate plus pyruvate.
引用
收藏
页码:161 / 168
页数:8
相关论文
共 37 条
[31]   ANAEROBIC DEGRADATION OF 2-FLUOROBENZOATE BY BENZOATE-DEGRADING, DENITRIFYING BACTERIA [J].
SCHENNEN, U ;
BRAUN, K ;
KNACKMUSS, HJ .
JOURNAL OF BACTERIOLOGY, 1985, 161 (01) :321-325
[32]  
SEMLER U, 1987, Z NATURFORSCH C, V42, P1070
[33]  
STOCKIGT J, 1975, Z NATURFORSCH C, V30, P352
[34]   PURIFICATION AND PROPERTIES OF 3 TYPES OF MONOHYDROXYBENZOATE OXYGENASE FROM RHODOCOCCUS-ERYTHROPOLIS S-1 [J].
SUEMORI, A ;
KURANE, R ;
TOMIZUKA, N .
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1993, 57 (09) :1487-1491
[35]  
VITOVSKI S, 1993, FEMS MICROBIOL LETT, V108, P1
[36]  
WEBSTER LT, 1973, J BIOL CHEM, V249, P2641
[37]   ACTIVATION OF AROMATIC-ACIDS AND AEROBIC 2-AMINOBENZOATE METABOLISM IN A DENITRIFYING PSEUDOMONAS STRAIN [J].
ZIEGLER, K ;
BUDER, R ;
WINTER, J ;
FUCHS, G .
ARCHIVES OF MICROBIOLOGY, 1989, 151 (02) :171-176