OBSERVATION OF NONCOVALENT COMPLEXES TO THE AVIDIN TETRAMER BY ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY

被引：58

作者：

SCHWARTZ, BL ^{[1
]}

LIGHTWAHL, KJ ^{[1
]}

SMITH, RD ^{[1
]}

机构：

[1] PACIFIC NW LAB, DEPT CHEM SCI, CHEM METHODS & SEPARAT GRP, RICHLAND, WA 99352 USA

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 1994年 / 5卷 / 03期

关键词：

D O I：

10.1016/1044-0305(94)85034-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Intact avidin-biotin and avidin-biotin maleimide noncovalent complexes have been observed by electrospray ionization mass spectrometry (ESI-MS) by using an extended mass range quadrupole mass spectrometer. By utilizing mild ESI interface conditions, the expected solution behavior of four biotin or biotin maleimide molecules noncovalently binding to each avidin tetramer can be preserved in the gas phase. The ESI-MS results show the appropriate mass additions of 973 +/- 60 Da for biotin and 1802 +/- 40 Da for biotin maleimide to the avidin tetramer species. These results support the hypothesis that substantial retention of higher order structure is possible in the gas phase by using gentle ESI conditions.

引用

页码：201 / 204

页数：4

共 24 条

[21] SMITH PB, 1993, 41ST P ASMS C MASS S, pA76
[22] PRESERVATION OF NONCOVALENT ASSOCIATIONS IN ELECTROSPRAY IONIZATION MASS-SPECTROMETRY - MULTIPLY CHARGED POLYPEPTIDE AND PROTEIN DIMERS
SMITH, RD
LIGHTWAHL, KJ
WINGER, BE
LOO, JA
[J]. ORGANIC MASS SPECTROMETRY, 1992, 27 (07): : 811 - 821
[23] THE OBSERVATION OF NONCOVALENT INTERACTIONS IN SOLUTION BY ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY - PROMISE, PITFALLS AND PROGNOSIS
SMITH, RD
LIGHTWAHL, KJ
[J]. BIOLOGICAL MASS SPECTROMETRY, 1993, 22 (09) : 493 - 501
[24] OBSERVATION AND IMPLICATIONS OF HIGH MASS-TO-CHARGE RATIO IONS FROM ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY
WINGER, BE
LIGHTWAHL, KJ
LOO, RRO
UDSETH, HR
SMITH, RD
[J]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1993, 4 (07) : 536 - 545

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