EXPRESSION AND CHARACTERIZATION OF GLYCOPROTEIN GP35 OF HEPATITIS-C VIRUS USING RECOMBINANT VACCINIA VIRUS

被引：33

作者：

KOHARA, M

TSUKIYAMAKOHARA, K

MAKI, N

ASANO, K

YAMAGUCHI, K

MIKI, K

TANAKA, S

HATTORI, N

MATSUURA, Y

SAITO, I

MIYAMURA, T

NOMOTO, A

机构：

[1] TOKYO METROPOLITAN KOMAGOME HOSP,BUNKYO KU,TOKYO 113,JAPAN

[2] NATL INST HLTH,DEPT ENTEROVIRUSES,SHINAGAWA KU,TOKYO 141,JAPAN

[3] NATL INST HLTH,DEPT VET SCI,SHINAGAWA KU,TOKYO 141,JAPAN

[4] TOKYO METROPOLITAN GERIATR HOSP & INST GERONTOL,DEPT MICROBIOL,TOKYO 173,JAPAN

来源：

JOURNAL OF GENERAL VIROLOGY | 1992年 / 73卷

关键词：

D O I：

10.1099/0022-1317-73-9-2313

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Complementary DNA clones corresponding to one of the putative structural regions of the hepatitis C virus (HCV) genome were obtained from sera of non-A non-B hepatitis patients. The putative envelope gene was expressed by using a recombinant vaccinia virus carrying this region of the HCV genome. In cells infected with the recombinant vaccinia virus, a glycosylated protein with an M(r) of about 35K (gp35) was specifically detected by convalescent sera from hepatitis C patients. The sera from rabbits immunized with this recombinant vaccinia virus reacted to the gp35 produced in insect cells and also to gp35 which was translated in vitro in the glycosylated and processed form. The gp35 was used to detect antibodies in sera of only 7 to 23 % of HCV patients at various stages of HCV disease. These results suggest that the gp35 of HCV may not have high antigenicity in humans.

引用

页码：2313 / 2318

页数：6

共 21 条

[1] SERODIAGNOSIS OF HEPATITIS-C VIRUS (HCV) INFECTION WITH AN HCV CORE PROTEIN MOLECULARLY EXPRESSED BY A RECOMBINANT BACULOVIRUS [J].