MUTATION DETECTION USING IMMOBILIZED MISMATCH BINDING-PROTEIN (MUTS)

被引：63

作者：

WAGNER, R ^{[1
]}

DEBBIE, P ^{[1
]}

RADMAN, M ^{[1
]}

机构：

[1] INST JACQUES MONOD,F-75251 PARIS 05,FRANCE

来源：

NUCLEIC ACIDS RESEARCH | 1995年 / 23卷 / 19期

关键词：

D O I：

10.1093/nar/23.19.3944

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An accurate and highly sensitive mutation detection assay has been developed. The assay is based on the detection of mispaired and unpaired bases by immobilized mismatch binding protein (Escherichia coli MutS). The assay can detect most mismatches and all single base substitution mutations, as well as small addition or deletion mutations. The assay is simple to use and does not require the use of either radioactivity or gel electrophoresis.

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页码：3944 / 3948

页数：5

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