DETECTION OF PLASMID TRANSFER FROM PSEUDOMONAS-FLUORESCENS TO INDIGENOUS BACTERIA IN SOIL BY USING BACTERIOPHAGE PHI-R2F FOR DONOR COUNTERSELECTION

被引:78
作者
SMIT, E [1 ]
VANELSAS, JD [1 ]
VANVEEN, JA [1 ]
DEVOS, WM [1 ]
机构
[1] AGR UNIV WAGENINGEN,DEPT MICROBIOL,6703 CT WAGENINGEN,NETHERLANDS
关键词
D O I
10.1128/AEM.57.12.3482-3488.1991
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The transfer of a genetically marked derivative of plasmid RP4, RP4p, from Pseudomonas fluorescens to members of the indigenous microflora of the wheat rhizosphere was studied by using a bacteriophage that specifically lyses the donor strain and a specific eukaryotic marker on the plasmid. Transfer of RP4p to the wheat rhizosphere microflora was observed, and the number of transconjugants detected was approximately 10(3) transconjugants per g of soil when 10(7) donor cells per g of soil were added; transfer in the corresponding bulk soil was slightly above the limit of detection. All of the indigenous transconjugants which we analyzed contained a 60-kb plasmid and were able to transfer this plasmid to a Nx(r) Rp(r) P. fluorescens recipient strain. The indigenous transconjugants were identified as belonging to Pseudomonas spp., Enterobacter spp., Comamonas spp., and Alcaligenes spp.
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收藏
页码:3482 / 3488
页数:7
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