EFFECTS OF TRANSFORMING GROWTH FACTOR-BETA-1 ON ASTROGLIAL CELLS IN CULTURE

被引：84

作者：

TORUDELBAUFFE, D

BAGHDASSARIANCHALAYE, D

GAVARET, JM

COURTIN, F

POMERANCE, M

PIERRE, M

机构：

[1] INSERM U. 96, Hôpital Kremlin-Bicêtre, Le Kremlin-Bicetre

来源：

JOURNAL OF NEUROCHEMISTRY | 1990年 / 54卷 / 03期

关键词：

Astroglial cells; Transforming growth factor β‐DNA synthesis–Glutamine synthetase activity;

D O I：

10.1111/j.1471-4159.1990.tb02357.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract: The effects of transforming growth factor β1 (TGFβ1) on DNA synthesis and functional differentiation of astroglial cells cultured in serum‐free medium were investigated. TGFβ1 diminished and delayed the peak of DNA synthesis induced by serum. TGFβ1‐treated cells were larger than control cells. This factor delayed the appearance of process‐bearing cells induced by acidic fibroblast growth factor treatment and also affected the astrocyte‐specific enzyme glutamine synthetase (GS), whose accumulation is under hydrocortisone (HC) control. TGFβ1 inhibited the induction of GS activity by HC in a dose‐ and time‐dependent manner. Moreover, pretreatment with TGFβ1 for 4 h maintained the inhibition of GS activity for ˜16 h after removal of this factor from culture medium. These results suggest that TGFβ1 may be an important regulator of astrocyte growth and differentiation. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：1056 / 1061

页数：6

共 39 条

[11] PURIFICATION OF A FACTOR THAT PROMOTES NEURITE OUTGROWTH - ISOLATION OF LAMININ AND ASSOCIATED MOLECULES [J].