EPITHELIAL CELL-FIBROBLAST INTERACTIONS - MODULATION OF EXTRACELLULAR-MATRIX PROTEINS IN CULTURED ORAL CELLS

被引:11
作者
HOU, LT [1 ]
KOLLAR, EJ [1 ]
YAEGER, JA [1 ]
机构
[1] UNIV CONNECTICUT,SCH DENT MED,DEPT BIOSTRUCT & FUNCT,FARMINGTON,CT 06032
关键词
EXTRACELLULAR MATRIX PROTEINS; FIBROBLASTS; EPITHELIAL ROOT SHEATH; GINGIVAL EPITHELIUM;
D O I
10.1111/j.1600-0765.1993.tb01057.x
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
A model system involving co-cultures of human gingival or periodontal ligament fibroblasts with mouse epithelial root sheath cells or human gingival epithelial cells was used to study epithelial cell-fibroblast interactions. Double-labeled immunofluorescence and microfluorometry were used to investigate the expression of extracellular matrix molecules of collagen type I (collagen I), type III (collagen III) and fibronectin in fibroblasts. When fibroblasts from either source were cultured alone, the fluorescence for collagen I and fibronectin ranged from strongly positive to almost negative. Collagen III staining was relatively weak compared with that of collagen I. After 2-3 days of co-culture, gingival fibroblasts and ligament fibroblasts adjacent to the mouse sheath cells exhibited enhanced intracellular fluorescence for collagen I and fibronectin. Very little change was observed for collagen III. Gingival fibroblasts cultured with gingival epithelial cells showed increased fluorescence for collagen I but decreased fluorescence for fibronectin. In contrast, the fluorescence intensity for both collagen I and fibronectin in ligament fibroblasts were reduced after 3 days of co-culture with gingival epithelial cells. Ultrastructural changes in fibroblasts co-cultured with mouse root sheath cells included increased Golgi cisternae and vesicles and an increased abundance of rough endoplasmic reticulum, polyribosomes, secretory vesicles and pinocytotic vesicles. Thus, the expression of extracellular matrix proteins and the metabolic activity of fibroblasts can be modulated by oral epithelial cells.
引用
收藏
页码:102 / 114
页数:13
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