INVESTIGATION OF GLIADIN BINDING TO DIFFERENT SELECTED PROTEINS USING A BIOTIN-STREPTAVIDIN SYSTEM

被引：10

作者：

OSMAN, AA ^{[1
]}

BRAUNERSREUTHER, I ^{[1
]}

MOTHES, T ^{[1
]}

机构：

[1] UNIV LEIPZIG,DEPT MED,INST PATHOL BIOCHEM,LIEBIGSTR 16,D-04103 LEIPZIG,GERMANY

来源：

ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG | 1994年 / 198卷 / 03期

关键词：

D O I：

10.1007/BF01192604

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Using a biotin streptavidin system 12 out of 19 different test proteins were found to bind to gliadin, with casein, fibrinogen, and fibronectin showing the strongest association. Binding was demonstrable using different gliadin subfractions and enzymatic digests. There was no consistent effect of several carbohydrates on binding. Binding of most proteins to gliadin was diminished slightly by removal of Ca2+ and strongly by dioxane, ethylene glycol, Triton X-100, and Tween 20. The results suggest a non-selective association between gliadin and the proteins investigated, mainly due to hydrophobic interactions, which might be of significance in intestinal pathobiochemistry, e. g. disease.

引用

页码：249 / 252

页数：4

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