OXYGEN RADICAL PRODUCTION IS INCREASED IN MACROPHAGES FROM DIABETES-PRONE BB-RATS

被引:26
作者
BRENNER, HH [1 ]
BURKART, V [1 ]
ROTHE, H [1 ]
KOLB, H [1 ]
机构
[1] UNIV DUSSELDORF,DIABET RES INST,HENNEKAMP 65,D-40225 DUSSELDORF,GERMANY
关键词
AUTOIMMUNITY; OXYGEN RADICALS; MACROPHAGES; BB-RAT; DIABETES;
D O I
10.3109/08916939309043883
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Macrophages from autoimmune diabetes prone BB rats were found to produce radical oxygen intermediates (ROI) at an enhanced rate when compared to diabetes resistant BB or normal Wistar rats. The release of ROI was determined by chemiluminescence using in parallel luminol and lucigenin as detector molecules. In diabetes prone BB rats the spontaneous release of ROI was upregulated in macrophages from different compartments, i.e. peritoneum and spleen. Also, maximal output of ROI after activation of macrophages either in vivo by injection of Corynebacterium parvum or in vitro by LPS and IFN was highest for cells from diabetes prone BB rats. This macrophage abnormality was seen in animals prior to recognizable islet inflammation and also was present at the level of macrophages grown in vitro from precursor cells of diabetes prone BB rats. Hypersecretion of oxygen radicals may contribute to Beta cell loss and diabetes development in BB rats.
引用
收藏
页码:93 / 98
页数:6
相关论文
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