SILVER ENHANCEMENT OF NICKEL-DIAMINOBENZIDINE AS APPLIED TO SINGLE AND DOUBLE IMMUNOPEROXIDASE STAINING

被引：18

作者：

FRIGO, B

SCOPSI, L

PATRIARCA, C

RILKE, F

机构：

[1] Division of Anatomical Pathology and Cytology, Istituto Nazionale per lo Studio e la Cura dei Tumori, Milan

来源：

BIOTECHNIC & HISTOCHEMISTRY | 1991年 / 66卷 / 03期

关键词：

IMMUNOCYTOCHEMISTRY; DETECTION EFFICIENCY; DIAMINOBENZIDINE; SILVER ENHANCEMENT; ARGYROPHILIA;

D O I：

10.3109/10520299109110572

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A reliable and practical method is proposed for increasing sensitivity and detection efficiency of immunocytochemical techniques, based on silver enhancement of the nickel-diaminobenzidine product of the peroxidase reaction. The procedure produces a strong signal at the site of the end product of the peroxidase reaction which is visible as black grains at the light microscopic level. The method has been used to detect peroxidase labeled probes in immunocytochemical tissue preparations and blotting assays and is ideal for the purposes of double staining and photographic documentation.

引用

页码：159 / 166

页数：8

共 27 条

[21]

SCOPSI L, 1986, MED BIOL, V64, P139

[22] INCREASED SENSITIVITY IN PEROXIDASE IMMUNOCYTOCHEMISTRY - A COMPARATIVE-STUDY OF A NUMBER OF PEROXIDASE VISUALIZATION METHODS EMPLOYING A MODEL SYSTEM [J].