PREVALENCE OF GROUP-A AND GROUP-B ROTAVIRUSES IN THE FECS OF NEONATAL DAIRY CALVES FROM CALIFORNIA

被引：17

作者：

CHINSANGARAM, J ^{[1
]}

SCHORE, CE ^{[1
]}

GUTERBOCK, W ^{[1
]}

WEAVER, LD ^{[1
]}

OSBURN, BI ^{[1
]}

机构：

[1] UNIV CALIF DAVIS,SCH VET MED,CTR VET MED TEACHING & RES,DAVIS,CA 95616

来源：

COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES | 1995年 / 18卷 / 02期

关键词：

GROUP-A BOVINE ROTAVIRUS; GROUP-B BOVINE ROTAVIRUS; ROTAVIRAL DIARRHEA; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION (RT-PCR); POLYACRYLAMIDE GEL ELECTROPHORESIS (PAGE); SHORT ELECTROPHEROTYPE;

D O I：

10.1016/0147-9571(95)98850-H

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

136 fecal samples, collected from 47 dairy calves on a calf ranch and in a dairy herd in California, were tested for the presence of group A and group B rotaviruses by reverse transcription-polymerase chain reaction (RT-PCR). Samples were collected from each calf at days 1, 7 and 14. Within the 14 day period, 44 calves (94%) were positive for group A rotavirus and an unexpectedly high number of calves (38 calves, 81%) were positive for group B rotavirus. When these samples were examined by polyacrylamide gel electrophoresis (PAGE), rotavirus was found in 21 calves and all of them had group A electropherotype. Among 25 PAGE positive samples from 21 calves, 17 (68%) were of short electropherotype, 4 (28%) were of long electropherotype and 4 (28%) contained both short and long electropherotype rotaviruses. Group B and short and long electropherotype group A rotaviruses were found in both normal and diarrheic calves.

引用

页码：93 / 103

页数：11

共 31 条

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