A SIMPLE DNA DIAGNOSTIC METHOD FOR HUMAN GENETIC-DISORDERS

被引：10

作者：

RABIN, D ^{[1
]}

DATTAGUPTA, N ^{[1
]}

机构：

[1] MOLEC DIAGNOST INC, 400 MORGAN LANE, W HAVEN, CT 06516 USA

来源：

HUMAN GENETICS | 1987年 / 75卷 / 02期

关键词：

D O I：

10.1007/BF00591071

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A fast, reliable, and simple technique for detecting point mutations in unfractionated human DNA has been developed. Oligonucleotide probes complementary to either sickle- or normal .beta.-globin DNA are labeled by primer extension and hybridized to DNA applied to nitrocellulose paper in a dot-blot format. A short hybridization time (about 1 h) and low probe concentration (about 1 nM) yield low background and high specificity. Double-blind trials show 100% agreement with restriction fragment length polymorphism (RFLP) analysis of DNA from normal, sickle, and heterozygous subjects.

引用

页码：120 / 122

页数：3

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