IDENTIFICATION OF A NEW ICE-NUCLEATING BACTERIUM AND ITS ICE NUCLEATION PROPERTIES

被引：29

作者：

OBATA, H ^{[1
]}

TAKINAMI, K ^{[1
]}

TANISHITA, J ^{[1
]}

HASEGAWA, Y ^{[1
]}

KAWATE, S ^{[1
]}

TOKUYAMA, T ^{[1
]}

UENO, T ^{[1
]}

机构：

[1] TOTTORI UNIV, DEPT AGR CHEM, TOTTORI 680, JAPAN

来源：

AGRICULTURAL AND BIOLOGICAL CHEMISTRY | 1990年 / 54卷 / 03期

关键词：

D O I：

10.1080/00021369.1990.10870030

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A new ice-nucleating bacterium, KUEN-3, was isolated from strawberry leaves. The ice-nucleating bacterium was found in the pale yellow colony group. Strain KUIN-3 was identified as Erwinia uredovora from its taxonomical characteristics. When strain KUIN-3 was cultured aerobically in a medium consisting of Simmons-citric acid broth (pH 6.1) for 24 hr at 18°C, the ice-nucleating activity of strain KUIN-3 cells was obtained. The ice-nucleating temperature, T50 (°C) was detected at — 2.8°C in cell suspensions (1.1 χ IO8 cells/ml) of strain KUIN-3. The ice-nucleating temperature was significantly decreased by treating cell suspensions for 30 min at 40°C and was completely lost by heating them at 90°C. The plot of ice-nucleating temperature versus treatment temperature (°C) is similar to the curve that was obtained for cell suspensions of another ice-nucleating bacteria. The susceptibility of cell ice nuclei to urea or protein-modifying reagents suggests that proteins participate in the ice-nucleating event. © 1990 by the Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.

引用

页码：725 / 730

页数：6

共 20 条

[11] ICE NUCLEATION INDUCED BY PSEUDOMONAS-SYRINGAE [J].