EVIDENCE THAT THE BLOCKADE OF MITOCHONDRIAL RESPIRATION BY THE NEUROTOXIN 1-METHYL-4-PHENYLPYRIDINIUM (MPP+) INVOLVES BINDING AT THE SAME SITE AS THE RESPIRATORY INHIBITOR, ROTENONE

被引：70

作者：

KRUEGER, MJ

SINGER, TP

CASIDA, JE

RAMSAY, RR

机构：

[1] VET ADM MED CTR,DIV MOLEC BIOL,SAN FRANCISCO,CA 94121

[2] UNIV CALIF SAN FRANCISCO,DIV TOXICOL,SAN FRANCISCO,CA 94143

[3] UNIV CALIF BERKELEY,DEPT ENTOMOL,BERKELEY,CA 94720

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 169卷 / 01期

关键词：

D O I：

10.1016/0006-291X(90)91442-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It has been postulated that 1-methyl-4-phenylpyridinium (MPP+) blocks mitochondrial respiration by combining at the same site as rotenone, a potent inhibitor of NADH oxidation in mitochondria, known to act at the junction of NADH dehydrogenase and coenzyme Q (CoQ). The present experiments show that MPP+ and two of its analogs indeed act in a concentration dependent manner to prevent the binding of [14C]-rotenone to submitochondrial particles (ETP) and significantly decrease the inhibition of electron transport caused by rotenone. It therefore appears that MPP+ binds at the same site as rotenone or an adjacent site, supporting the hypothesis that its neurotoxic action is due to the inhibition of mitochondrial respiration. © 1990.

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页码：123 / 128

页数：6

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