SAMPLE GATING IN OPEN TUBULAR AND PACKED CAPILLARIES FOR HIGH-SPEED LIQUID-CHROMATOGRAPHY

被引：24

作者：

MONNIG, CA ^{[1
]}

DOHMEIER, DM ^{[1
]}

JORGENSON, JW ^{[1
]}

机构：

[1] UNIV N CAROLINA,DEPT CHEM,CHAPEL HILL,NC 27599

来源：

ANALYTICAL CHEMISTRY | 1991年 / 63卷 / 08期

关键词：

D O I：

10.1021/ac00008a014

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new liquid chromatography instrument is described that employs small-diameter capillary columns and an optically controlled sample gating procedure to separate a mixture of fluorescein isothiocyanate (FITC) labeled amines in as few as 6 s. Efficiency of the separation, expressed as the number of theoretical plates, is observed to increase linearly with column length and thus analysis time. The combination of short analysis time and automated sample introduction allows signal averaging to be used to enhance the precision of the measurement.

引用

页码：807 / 810

页数：4

共 6 条

[1] NEW METHOD FOR THE PREPARATION OF SMALL DIAMETER COLUMNS WITH POLYMERIC STATIONARY PHASES FOR OPEN TUBULAR LIQUID-CHROMATOGRAPHY [J].