EXPRESSION OF RAT 5-ALPHA-REDUCTASE IN SACCHAROMYCES-CEREVISIAE

被引：12

作者：

ORDMAN, AB ^{[1
]}

FARLEY, D ^{[1
]}

MEYHACK, B ^{[1
]}

NICK, H ^{[1
]}

机构：

[1] CIBA GEIGY AG,PHARMACEUT RES,CH-4002 BASEL,SWITZERLAND

来源：

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1991年 / 39卷 / 04期

关键词：

D O I：

10.1016/0960-0760(91)90242-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dihydrotestosterone (DHT) is the principle androgen in certain tissues such as the prostate. DHT is formed from testosterone by the NADPH-dependent enzyme 5-alpha-reductase (5AR). In this paper we report the expression of catalytically active steroid 5AR from the rat in Saccharomyces cerevisiae. A full length cDNA coding for 5AR was isolated from a rat liver cDNA library and fixed in frame to the signal sequence of yeast acid phosphatase. A constitutive short promoter fragment of the acid phosphatase gene (PHO 5) and the PHO 5 transcriptional terminator were added and the expression cassette ligated into the yeast 2-mu-vector pDP34. S. cerevisiae transformed with the 5AR expression plasmid pDP34/PHO5AR exhibited about 100-fold more activity per gram wet weight than rat prostate.

引用

页码：487 / 492

页数：6

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