EXPRESSION OF RAT 5-ALPHA-REDUCTASE IN SACCHAROMYCES-CEREVISIAE

被引：12

作者：

ORDMAN, AB ^{[1
]}

FARLEY, D ^{[1
]}

MEYHACK, B ^{[1
]}

NICK, H ^{[1
]}

机构：

[1] CIBA GEIGY AG,PHARMACEUT RES,CH-4002 BASEL,SWITZERLAND

来源：

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1991年 / 39卷 / 04期

关键词：

D O I：

10.1016/0960-0760(91)90242-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dihydrotestosterone (DHT) is the principle androgen in certain tissues such as the prostate. DHT is formed from testosterone by the NADPH-dependent enzyme 5-alpha-reductase (5AR). In this paper we report the expression of catalytically active steroid 5AR from the rat in Saccharomyces cerevisiae. A full length cDNA coding for 5AR was isolated from a rat liver cDNA library and fixed in frame to the signal sequence of yeast acid phosphatase. A constitutive short promoter fragment of the acid phosphatase gene (PHO 5) and the PHO 5 transcriptional terminator were added and the expression cassette ligated into the yeast 2-mu-vector pDP34. S. cerevisiae transformed with the 5AR expression plasmid pDP34/PHO5AR exhibited about 100-fold more activity per gram wet weight than rat prostate.

引用

页码：487 / 492

页数：6

共 17 条

[11] INHIBITORS OF STEROID 5 ALPHA-REDUCTASE IN BENIGN PROSTATIC HYPERPLASIA, MALE PATTERN BALDNESS AND ACNE [J].

METCALF, BW ;

LEVY, MA ;

HOLT, DA .

TRENDS IN PHARMACOLOGICAL SCIENCES, 1989, 10 (12) :491-495

[12]

MEYHACK B, 1989, GENETICS MOL BIOL IN, P311

[13] EXPRESSION OF RAT-LIVER CYTOCHROME P-450MC CDNA IN SACCHAROMYCES-CEREVISIAE [J].

OEDA, K ;

SAKAKI, T ;

OHKAWA, H .

DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1985, 4 (03) :203-210

[14] AZASTEROIDS - STRUCTURE-ACTIVITY-RELATIONSHIPS FOR INHIBITION OF 5-ALPHA-REDUCTASE AND OF ANDROGEN RECEPTOR-BINDING [J].

RASMUSSON, GH ;

REYNOLDS, GF ;

STEINBERG, NG ;

WALTON, E ;

PATEL, GF ;

LIANG, TM ;

CASCIERI, MA ;

CHEUNG, AH ;

BROOKS, JR ;

BERMAN, C .

JOURNAL OF MEDICINAL CHEMISTRY, 1986, 29 (11) :2298-2315

[15]

Sambrook J., 1989, MOL CLONING LAB MANU

[16] EXPRESSION OF UDP-GLUCURONOSYLTRANSFERASE CDNA IN SACCHAROMYCES-CEREVISIAE AS A MEMBRANE-BOUND AND AS A CYTOSOLIC FORM [J].

TOGHROL, F ;

KIMURA, T ;

OWENS, IS .

BIOCHEMISTRY, 1990, 29 (09) :2349-2356

[17] SEXUAL DIFFERENTIATION [J].

WILSON, JD .

ANNUAL REVIEW OF PHYSIOLOGY, 1978, 40 :279-306

← 1 2 →