FUNCTIONAL-ANALYSIS OF CDNAS FOR 2 TYPES OF HUMAN HEME OXYGENASE AND EVIDENCE FOR THEIR SEPARATE REGULATION

被引：182

作者：

SHIBAHARA, S ^{[1
]}

YOSHIZAWA, M ^{[1
]}

SUZUKI, H ^{[1
]}

TAKEDA, K ^{[1
]}

MEGURO, K ^{[1
]}

ENDO, K ^{[1
]}

机构：

[1] TOHOKU UNIV,SCH MED,DEPT INTERNAL MED 2,AOBA KU,SENDAI,MIYAGI 980,JAPAN

来源：

JOURNAL OF BIOCHEMISTRY | 1993年 / 113卷 / 02期

关键词：

D O I：

10.1093/oxfordjournals.jbchem.a124028

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We cloned a cDNA coding for a putative human heme oxygenase isozyme, designated type 2 (HO-2), and analyzed its function by transient expression assays. HeLa cells transfected with either HO-2 cDNAs or a cDNA coding for authentic heme oxygenase (HO-1) expressed the activity of heme oxygenase, although no activity was detected in the mock transfected cells. Using specific anti-HO-1 antibody, we showed that expression of a HO-1 cDNA resulted in the increase in its protein levels, but HO-1 protein was not detectable in the cells expressing HO-2 cDNAs. We thus confirmed the functional identity of HO-1 and HO-2. Then, we analyzed their expression in an erythroid cell line, YN-1-0-A. Treatment with hemin or by heat shock (42-degrees-C) led to a remarkable increase in the HO-1 mRNA levels, while HO-2 mRNA expression was not induced at all, suggesting that they are under separate regulation.

引用

页码：214 / 218

页数：5

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