COMPARATIVE GENOMIC HYBRIDIZATION FOR MOLECULAR CYTOGENETIC ANALYSIS OF SOLID TUMORS

被引：2517

作者：

KALLIONIEMI, A ^{[1
]}

KALLIONIEMI, OP ^{[1
]}

SUDAR, D ^{[1
]}

RUTOVITZ, D ^{[1
]}

GRAY, JW ^{[1
]}

WALDMAN, F ^{[1
]}

PINKEL, D ^{[1
]}

机构：

[1] WESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLAND

来源：

SCIENCE | 1992年 / 258卷 / 5083期

关键词：

D O I：

10.1126/science.1359641

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Comparative genomic hybridization produces a map of DNA sequence copy number as a function of chromosomal location throughout the entire genome. Differentially labeled test DNA and normal reference DNA are hybridized simultaneously to normal chromosome spreads. The hybridization is detected with two different fluorochromes. Regions of gain or loss of DNA sequences, such as deletions, duplications, or amplifications, are seen as changes in the ratio of the intensities of the two fluorochromes along the target chromosomes. Analysis of tumor cell lines and primary bladder tumors identified 16 different regions of amplification, many in loci not previously known to be amplified.

引用

页码：818 / 821

页数：4