LONG-TERM CHANGES IN REGIONAL BRAIN CYTOCHROME-OXIDASE ACTIVITY INDUCED BY ELECTROCONVULSIVE TREATMENT IN RATS

Quantitative cytochrome oxidase (CO) histochemistry was used to examine brain regional metabolic effects of electroconvulsive shock-induced seizures (ECS). Rats received a course of either eight ECS or control treatments and were sacrificed either 24 h or 28 days after the last session. Regional CO activity (mumol/gT/min) was quantitated throughout the brain using internally calibrated standards. Twenty-four hours after the last ECS session there was no significant difference between ECS- and sham-treated brains in any of the 99 brain regions examined. In contrast, 28 days after the last session, ECS brains showed significant increases in CO activity in the interpeduncular nucleus (+20%), bed nucleus of the stria terminalis (+25%), dorsomedial hypothalamus (+20%), ventromedial hypothalamus (+12%), mammillary nucleus (+14%), pontine nucleus (+16%), basolateral amygdala (+14%), medial amygdala (+12%), piriform cortex (+12%) and ventromedial thalamus (+9%). These results suggest that ECS induces localized increases in brain CO activity which are long-lasting and may develop independently of additional stimulation. The fact that CO changes were predominantly in limbic areas suggests that they may be relevant to the antidepressant effects of ECS.