STUDIES ON PLANT-TISSUE CULTURES .66. BIOTRANSFORMATION OF DIGITOXIGENIN BY GINSENG HAIRY ROOT CULTURES

被引：31

作者：

KAWAGUCHI, K ^{[1
]}

HIROTANI, M ^{[1
]}

YOSHIKAWA, T ^{[1
]}

FURUYA, T ^{[1
]}

机构：

[1] KITASATO UNIV,SCH PHARMACEUT SCI,MINATO KU,TOKYO 108,JAPAN

来源：

PHYTOCHEMISTRY | 1990年 / 29卷 / 03期

关键词：

Araliaceae; biotransformation; cardenolides; digitoxigenin; digitoxigenin palmitate; β-D-sophoroside; esterification; glycosylation; hairy root cultures; Panax ginseng;

D O I：

10.1016/0031-9422(90)80029-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Five new compounds (three esters and two glycosides) and seven previously reported compounds were isolated as biotransformation products of digitoxigenin by ginseng hairy root cultures. The new esters and glycosides were elucidated as digitoxigenin stearate, digitoxigenin palmitate, digitoxigenin myristate, 3-epidigitoxigenin β-D- gentiobioside and digitoxigenin β-D-sophoroside using 1H and 13C NMR and FAB mass spectral data. Biotransformations involving esterification (of stearic acid, palmitic acid, myristic acid and lauric acid) and glycosylation (of gentiobiose and sophorose) of digitoxigenin have been demonstrated for the first time in the plant cell and tissue cultures. The hairy roots showed high glycosylation ability to the digitoxigenin molecule. © 1990.

引用

页码：837 / 843

页数：7