PHOSPHORYLATION OF THE TRANSCRIPTION FACTOR-PHO4 BY A CYCLIN-CDK COMPLEX, PHO80-PHO85

被引：335

作者：

KAFFMAN, A

HERSKOWITZ, I

TJIAN, R

OSHEA, EK

机构：

[1] UNIV CALIF SAN FRANCISCO, SCH MED, DEPT BIOCHEM & BIOPHYS, SAN FRANCISCO, CA 94143 USA

[2] UNIV CALIF BERKELEY, HOWARD HUGHES MED INST, DEPT MOLEC & CELL BIOL, BERKELEY, CA 94720 USA

来源：

SCIENCE | 1994年 / 263卷 / 5150期

关键词：

D O I：

10.1126/science.8108735

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Induction of the yeast gene PHO5 is mediated by the transcription factors PHO2 and PHO4. PHO5 transcription is not detectable in high phosphate; it is thought that the negative regulators PHO80 and PHO85 inactivate PHO2 and PHO4. Here it is reported that PHO80 has homology to yeast cyclins and interacts with PHO85, a p34cdc2/CDC28-related protein kinase. The PHO80-PHO85 complex phosphorylates PHO4; this phosphorylation is correlated with negative regulation of PHO5. These results demonstrate the existence of a cyclin-cdk complex that is used for a regulatory process other than cell-cycle control and identify a physiologically relevant substrate for this complex.

引用

页码：1153 / 1156

页数：4

共 36 条

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