OXYGEN AFFINITIES OF METRONIDAZOLE-RESISTANT AND METRONIDAZOLE SENSITIVE STOCKS OF GIARDIA-INTESTINALIS

被引:39
作者
ELLIS, JE
WINGFIELD, JM
COLE, D
BOREHAM, PFL
LLOYD, D
机构
[1] UNIV WALES COLL CARDIFF, COLL CARDIFF,SCH PURE & APPL BIOL,MICROBIOL GRP, POB 915, CARDIFF CF1 3TL, WALES
[2] QUEENSLAND INST MED RES, BANCROFT CTR, BRISBANE, QLD 4029, AUSTRALIA
基金
英国惠康基金;
关键词
GIARDIA-INTESTINALIS; OXYGEN AFFINITIES; DRUG RESISTANCE; METRONIDAZOLE; PYRUVATE; FERREDOXIN OXIDOREDUCTASE;
D O I
10.1016/0020-7519(93)90095-G
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
The common protozoon, Giardia intestinalis, parasitizes the upper small intestine of man, and is often refractory to treatment by metronidazole. Defective oxygen-scavenging mechanisms have been implicated as a cause of metronidazole resistance of another flagellate Trichomonas vaginalis, where metronidazole is also the most common drug treatment. Oxygen consumption of six clinical isolates of G. intestinalis and one line selected for resistance to metronidazole was measured over 0-50 mum-O2 using an oxygen electrode open for gas exchange. At > 30 mum-O2, inhibition of respiration was demonstrated in all seven stocks. Apparent oxygen affinities (K(m)O2) were found to range from 0.5 to 5.2 mum-O2; however, isolates from patients who failed to respond to treatment with metronidazole did not have measurably defective O2-scavenging capabilities compared with metronidazole-sensitive isolates. These strains did, however, show elevated NADPH-oxidase activities compared with metronidazole-sensitive strains. Results indicate that biochemical mechanisms of drug resistance in G. intestinalis may be quite different from those operating in T. vaginalis.
引用
收藏
页码:35 / 39
页数:5
相关论文
共 30 条
  • [11] LINDMARK DG, 1973, J BIOL CHEM, V248, P7724
  • [12] THE PURIFICATION AND PROPERTIES OF 2 SOLUBLE REDUCED NICOTINAMIDE - ACCEPTOR OXIDOREDUCTASES FROM TRICHOMONAS-VAGINALIS
    LINSTEAD, DJ
    BRADLEY, S
    [J]. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1988, 27 (2-3) : 125 - 134
  • [13] LLOYD D, 1985, J GEN MICROBIOL, V131, P87
  • [14] Lloyd D., 1989, P1
  • [15] LLOYD D, 1983, Journal of Microbiological Methods, V1, P313, DOI 10.1016/0167-7012(83)90008-8
  • [16] INCOMPLETE ANAEROBIOSIS AS A CAUSE OF METRONIDAZOLE RESISTANCE
    MILNE, SE
    STOKES, EJ
    WATERWORTH, PM
    [J]. JOURNAL OF CLINICAL PATHOLOGY, 1978, 31 (10) : 933 - 935
  • [17] MORENO SNJ, 1984, J BIOL CHEM, V259, P8252
  • [18] MULLER M, 1983, SURGERY, V93, P165
  • [19] MULLER M, 1981, SCAND J INFECT DIS, P31
  • [20] MULLER M, 1980, AM J OBSTET GYNECOL, V138, P808