WEIGHING NAKED PROTEINS - PRACTICAL, HIGH-ACCURACY MASS MEASUREMENT OF PEPTIDES AND PROTEINS

被引：405

作者：

CHAIT, BT ^{[1
]}

KENT, SBH ^{[1
]}

机构：

[1] SCRIPPS RES INST, LA JOLLA, CA 92037 USA

来源：

SCIENCE | 1992年 / 257卷 / 5078期

关键词：

D O I：

10.1126/science.1411504

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Two new technologies have made the study of proteins by mass spectrometry straight-forward. Proteins with molecular masses of up to more than 100 kilodaltons can be analyzed at picomole sensitivities to give simple mass spectra corresponding to the intact molecule. This development has allowed unprecedented accuracy in the determination of the molecular weights of proteins. A number of "case studies" are used to present the revolutionary impact that these powerful new ways of looking at proteins are having on biological research.

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页码：1885 / 1894

页数：10

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