APPLICATION OF A POLYMORPHIC Y-MICROSATELLITE TO THE DETECTION OF POST BONE-MARROW TRANSPLANTATION CHIMERISM

被引：12

作者：

LO, YMD

NOAKES, L

ROUX, E

JEANNET, M

CHAPUIS, B

FLEMING, KA

WAINSCOAT, JS

机构：

[1] JOHN RADCLIFFE HOSP,DEPT HAEMATOL,LRF,MOLEC HAEMATOL UNIT,OXFORD OX3 9DU,ENGLAND

[2] JOHN RADCLIFFE HOSP,NUFFIELD DEPT CLIN MED,OXFORD OX3 9DU,ENGLAND

[3] JOHN RADCLIFFE HOSP,NUFFIELD DEPT PATHOL & BACTERIOL,OXFORD OX3 9DU,ENGLAND

[4] HOP CANTONAL UNIV GENEVA,GENEVA,SWITZERLAND

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 1995年 / 89卷 / 03期

基金：

英国惠康基金;

关键词：

BONE MARROW TRANSPLANTATION; PCR; Y MICROSATELLITES; CHIMERISM; DNA AMPLIFICATION;

D O I：

10.1111/j.1365-2141.1995.tb08380.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

A highly sensitive Y-microsatellite amplification system was developed for the detection of post bone marrow transplantation (BMT) chimaerism. The system is able to detect the DNA equivalent of a single male cell in a background of 10(5) female cells. For clinical applications it has the distinct advantage that the polymorphic nature of the PCR product allows the checking of the genuineness of a positive PCR signal reference to ts allelic type. This extra degree of precision enhances the accuracy of Y-PCR for clinical diagnosis.

引用

页码：645 / 649

页数：5

共 9 条

[1] PREVENTION OF PRE-PCR MIS-PRIMING AND PRIMER DIMERIZATION IMPROVES LOW-COPY-NUMBER AMPLIFICATIONS [J].