MODIFICATION OF BRASSICA SEED OIL BY ANTISENSE EXPRESSION OF A STEAROYL-ACYL CARRIER PROTEIN DESATURASE GENE

被引：279

作者：

KNUTZON, DS ^{[1
]}

THOMPSON, GA ^{[1
]}

RADKE, SE ^{[1
]}

JOHNSON, WB ^{[1
]}

KNAUF, VC ^{[1
]}

KRIDL, JC ^{[1
]}

机构：

[1] CALGENE INC, 1920 5TH ST, DAVIS, CA 95616 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 07期

关键词：

FATTY ACID BIOSYNTHESIS; LIPID METABOLISM; RAPESEED; SATURATED FATTY ACIDS;

D O I：

10.1073/pnas.89.7.2624

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Molecular gene transfer techniques have been used to engineer the fatty acid composition of Brassica rapa and Brassica napus (canola) oil. Stearoyl-acyl carrier protein (stearoyl-ACP) desaturase (EC 1.14.99.6) catalyzes the first desaturation step in seed oil biosynthesis, converting stearoyl-ACP to oleoyl-ACP. Seed-specific antisense gene constructs of B. rapa stearoyl-ACP desaturase were used to reduce the protein concentration and enzyme activity of stearoyl-ACP desaturase in developing rapeseed embryos during storage lipid biosynthesis. The resulting transgenic plants showed dramatically increased stearate levels in the seeds. A continuous distribution of stearate levels from 2% to 40% was observed in seeds of a transgenic B. napus plant, illustrating the potential to engineer specialized seed oil compositions.

引用

页码：2624 / 2628

页数：5

共 31 条

[1] PROPERTIES OF THE GLYCEROL ACYLATING ENZYMES IN MICROSOMAL PREPARATIONS FROM THE DEVELOPING SEEDS OF SAFFLOWER (CARTHAMUS-TINCTORIUS) AND TURNIP RAPE (BRASSICA-CAMPESTRIS) AND THEIR ABILITY TO ASSEMBLE COCOA-BUTTER TYPE FATS
BAFOR, M
STOBART, AK
STYMNE, S
[J]. JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1990, 67 (04) : 217 - 225
[2] GENETIC-ENGINEERING FOR PLANT OILS - POTENTIAL AND LIMITATIONS
BATTEY, JF
SCHMID, KM
OHLROGGE, JB
[J]. TRENDS IN BIOTECHNOLOGY, 1989, 7 (05) : 122 - 126
[3] FATTY-ACID COMPOSITION OF LEAF LIPIDS DETERMINED AFTER COMBINED DIGESTION AND FATTY-ACID METHYL-ESTER FORMATION FROM FRESH TISSUE
BROWSE, J
MCCOURT, PJ
SOMERVILLE, CR
[J]. ANALYTICAL BIOCHEMISTRY, 1986, 152 (01) : 141 - 145
[4] Downey R. K., 1983, In 'High and low erucic acid rapeseed oils' G[see FSTA (1984) 16 G12N599]., P1
[5] NUTRIENT REQUIREMENTS OF SUSPENSION CULTURES OF SOYBEAN ROOT CELLS
GAMBORG, OL
MILLER, RA
OJIMA, K
[J]. EXPERIMENTAL CELL RESEARCH, 1968, 50 (01) : 151 - +
[6] GRAEF GL, 1985, CROP SCI, V25, P1076, DOI 10.2135/cropsci1985.0011183X002500060043x
[7] A MUTANT GENOTYPE OF FLAX (LINUM-USITATISSIMUM L) CONTAINING VERY LOW-LEVELS OF LINOLENIC ACID IN ITS SEED OIL
GREEN, AG
[J]. CANADIAN JOURNAL OF PLANT SCIENCE, 1986, 66 (03) : 499 - 503
[8] ANTISENSE GENE THAT INHIBITS SYNTHESIS OF THE HORMONE ETHYLENE IN TRANSGENIC PLANTS
HAMILTON, AJ
LYCETT, GW
GRIERSON, D
[J]. NATURE, 1990, 346 (6281) : 284 - 287
[9] IMPROVING THE FATTY-ACID COMPOSITION OF SOYBEAN OIL
HAMMOND, EG
FEHR, WR
[J]. JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1984, 61 (11) : 1713 - 1716
[10] THE APPLICATION OF GENETIC-ENGINEERING TO OILSEED CROPS
KNAUF, VC
[J]. TRENDS IN BIOTECHNOLOGY, 1987, 5 (02) : 40 - 47

← 1 2 3 4 →