ANTI-AIDS AGENTS .19. NEOTRIPTERIFORDIN, A NOVEL ANTI-HIV PRINCIPLE FROM TRIPTERYGIUM-WILFORDII - ISOLATION AND STRUCTURAL ELUCIDATION

A new kaurane type diterpene lactone, neotripterifordin (1), has been isolated from the roots of Tripterygium wilfordii. The structure of 1 was elucidated by spectroscopic methods, which included the concerted application of a number of 2-D NMR techniques including H-1-H-1 COSY, phase-sensitive NOESY, HETCOR, and long-range HETCOR. Compound 1 showed anti-HIV replication activity in H9 lymphocyte cells with an EC(50) of 25 nM and TI of 125.