SEPARATION OF PROTEINS BY CONSECUTIVE SODIUM DODECYL-SULFATE (SDS) POLYACRYLAMIDE-GEL ELECTROPHORESIS AND REVERSED PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：7

作者：

KAPLAN, B

PRAS, M

机构：

[1] Heller Institute of Medical Research, Chaim Sheba Medical Center, Sackler Medical School, Tel-Aviv University, Tel-Aviv

来源：

BIOMEDICAL CHROMATOGRAPHY | 1991年 / 5卷 / 02期

关键词：

D O I：

10.1002/bmc.1130050209

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A procedure for the preparative separation of proteins was developed by using consecutively sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (SDS-PAGE) and reversed phase high performance liquid chromatography (HPLC). The proteins were separated by SDS-PAGE and afterwards extracted from the gel. The extracted proteins were separated from SDS and other small molecular weight contaminants on a Fractogel TSK HW-40 (F) column in acidic aqueous acetonitrile. The proteins eluted from the Fractogel column were fractionated by HPLC. The identity and purity of the recovered proteins was confirmed by SDS-PAGE analysis.

引用

页码：86 / 89

页数：4

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