OXYGEN RADICAL PRODUCTION DURING ISCHEMIA - REPERFUSION IN THE ISOLATED PERFUSED-RAT-LIVER AS MONITORED BY LUMINOL ENHANCED CHEMILUMINESCENCE

被引：53

作者：

OKUDA, M

IKAI, I

CHANCE, B

KUMAR, C

机构：

[1] UNIV PENN,SCH MED,DEPT BIOCHEM & BIOPHYS,PHILADELPHIA,PA 19104

[2] UNIV CITY SCI CTR,PHILADELPHIA,PA 19104

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 174卷 / 01期

关键词：

D O I：

10.1016/0006-291X(91)90508-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have applied the Luminol enhanced chemiluminescence technique to the isolated perfused rat liver during ischemia and reperfusion to monitor the production of oxygen radicals in tissue. Livers under perfusion with Luminol-containing buffer were subjected to 30 minutes of global ischemia followed by 60 minutes of reperfusion. Their chemiluminescence was continuously monitored to obtain the time course of oxygen radical production. Transient bursts of oxygen radical production were observed in the livers as indicated by chemiluminescence changes on reperfusion. Superoxide dismutase treatment abolished while catalase treatment enhanced the reperfusion-induced chemiluminescence transient. © 1991.

引用

页码：217 / 221

页数：5

共 11 条

[1] PHAGOCYTIC ACTIVATION OF A LUMINOL-DEPENDENT CHEMILUMINESCENCE IN RABBIT ALVEOLAR AND PERITONEAL MACROPHAGES [J].