DETECTION OF ANTIBODIES IN SERA FROM CHAGAS-DISEASE PATIENTS USING A TRYPANOSOMA-CRUZI IMMUNODOMINANT RECOMBINANT ANTIGEN

被引：20

作者：

PARANHOSBACALLA, GS

SANTOS, MRM

COTRIM, P

RASSI, A

JOLIVET, M

CAMARGO, ME

DASILVEIRA, JF

机构：

[1] ESCOLA PAULISTA MED,DEPT MICROBIOL IMMUNOL & PARASITOL,RUA BOTUCATU 862,BR-04023062 SAO PAULO,BRAZIL

[2] BIOMERIEUX,LYON,FRANCE

[3] BIOLAB DIAGNOST,SAO PAULO,BRAZIL

[4] UFG,FAC MED GOIAS,GOIANIA,GO,BRAZIL

[5] USP,INST MED TROP,SAO PAULO,BRAZIL

来源：

PARASITE IMMUNOLOGY | 1994年 / 16卷 / 03期

关键词：

CHAGAS DISEASE; RECOMBINANT ANTIGEN; IMMUNODOMINANT REPETITIVE ANTIGEN; ELISA; SERODIAGNOSIS;

D O I：

10.1111/j.1365-3024.1994.tb00336.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A Trypanosoma cruzi DNA fragment encoding an immunodominant repetitive antigen (H49) was subcloned into a protein purification and expressed system. Purified H49 peptide reacted specifically in an enzyme-linked immunosorbent assay (ELISA) with sera from T. cruzi-infected patients, but not with sera from patients with other parasitic diseases such as leishmaniasis and T. rangeli-infection. The H49 recombinant ELISA was able to detect specific antibodies in 84% of chronic chagasic serum samples tested. One of the major advantage of the recombinant ELISA for serodiagnosis of chronic Chagas' disease resides in its high specificity (100%). Our data suggest that recombinant peptides could provide a practical basis for specific diagnosis tests for Chagas' disease.

引用

页码：165 / 169

页数：5

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