A DROP-COLLAPSING TEST FOR SCREENING SURFACTANT-PRODUCING MICROORGANISMS

被引：279

作者：

JAIN, DK ^{[1
]}

COLLINSTHOMPSON, DL ^{[1
]}

LEE, H ^{[1
]}

TREVORS, JT ^{[1
]}

机构：

[1] UNIV GUELPH,DEPT ENVIRONM BIOL,GUELPH N1G 2W1,ONTARIO,CANADA

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1991年 / 13卷 / 04期

关键词：

BIOSURFACTANT; DROP COLLAPSE; MICROBIAL SCREENING;

D O I：

10.1016/0167-7012(91)90064-W

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive rapid method was devised for screening bacterial colonies that produce surfactants. Drops of cell suspensions of surfactant-producing colonies collapsed on an oil-coated surface. Drops of cell suspensions of colonies that did not produce, or produced very low concentrations of surfactants remained stable. The stability of drops was dependent on biosurfactant concentration and it correlated with surface tension but not with emulsifying activity. Microbial colonies grown in presence of carbohydrates or hydrocarbons could be readily screened for surfactant production by this method.

引用

页码：271 / 279

页数：9

共 17 条

[1] SURFACTIN A CRYSTALLINE PEPTIDELIPID SURFACTANT PRODUCED BY BACILLUS SUBTILIS - ISOLATION CHARACTERIZATION AND ITS INHIBITION OF FIBRIN CLOT FORMATION [J].

ARIMA, K ;

KAKINUMA, A ;

TAMURA, G .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1968, 31 (03) :488-&

[2] STUDIES ON MODE OF ACTION AND BIOGENESIS OF PHYTOTOXIN SYRINGOMYCIN [J].

BACKMAN, PA ;

DEVAY, JE .

PHYSIOLOGICAL PLANT PATHOLOGY, 1971, 1 (02) :215-+

[3] IDENTIFICATION AND CHARACTERIZATION OF A SOIL BACTERIUM WITH EXTRACELLULAR EMULSIFYING ACTIVITY [J].

BERG, G ;

SEECH, AG ;

LEE, H ;

TREVORS, JT .

JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-ENVIRONMENTAL SCIENCE AND ENGINEERING & TOXIC AND HAZARDOUS SUBSTANCE CONTROL, 1990, 25 (07) :753-764

[4] NATURE AND PROPERTIES OF A CYTOLYTIC AGENT PRODUCED BY BACILLUS-SUBTILIS [J].

BERNHEIMER, AW ;

AVIGAD, LS .

JOURNAL OF GENERAL MICROBIOLOGY, 1970, 61 :361-+

[5]

Davis J. B., 1967, PETROLEUM MICROBIOLO

[6] PSEUDOMONAS-AERUGINOSA BIOSURFACTANT PRODUCTION IN CONTINUOUS CULTURE WITH GLUCOSE AS CARBON SOURCE [J].

GUERRASANTOS, L ;

KAPPELI, O ;

FIECHTER, A .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1984, 48 (02) :301-305

[7] SURFACE-ACTIVE SUBSTANCES PRODUCED BY THIOBACILLUS THIOOXIDANS [J].

JONES, GE ;

STARKEY, RL .

JOURNAL OF BACTERIOLOGY, 1961, 82 (05) :788-&

[8] CHEMICAL AND PHYSICAL CHARACTERIZATION OF INTERFACIAL-ACTIVE LIPIDS FROM RHODOCOCCUS-ERYTHROPOLIS GROWN ON NORMAL-ALKANES [J].

KRETSCHMER, A ;

BOCK, H ;

WAGNER, F .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1982, 44 (04) :864-870

[9] DIRECT COLONY THIN-LAYER CHROMATOGRAPHY AND RAPID CHARACTERIZATION OF SERRATIA-MARCESCENS MUTANTS DEFECTIVE IN PRODUCTION OF WETTING AGENTS [J].

MATSUYAMA, T ;

SOGAWA, M ;

YANO, I .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1987, 53 (05) :1186-1188

[10]

MULLIGAN CN, 1984, J FERMENT TECHNOL, V62, P311

← 1 2 →