A DROP-COLLAPSING TEST FOR SCREENING SURFACTANT-PRODUCING MICROORGANISMS

被引：279

作者：

JAIN, DK ^{[1
]}

COLLINSTHOMPSON, DL ^{[1
]}

LEE, H ^{[1
]}

TREVORS, JT ^{[1
]}

机构：

[1] UNIV GUELPH,DEPT ENVIRONM BIOL,GUELPH N1G 2W1,ONTARIO,CANADA

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1991年 / 13卷 / 04期

关键词：

BIOSURFACTANT; DROP COLLAPSE; MICROBIAL SCREENING;

D O I：

10.1016/0167-7012(91)90064-W

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive rapid method was devised for screening bacterial colonies that produce surfactants. Drops of cell suspensions of surfactant-producing colonies collapsed on an oil-coated surface. Drops of cell suspensions of colonies that did not produce, or produced very low concentrations of surfactants remained stable. The stability of drops was dependent on biosurfactant concentration and it correlated with surface tension but not with emulsifying activity. Microbial colonies grown in presence of carbohydrates or hydrocarbons could be readily screened for surfactant production by this method.

引用

页码：271 / 279

页数：9

共 17 条

[11]

NEU TR, 1990, APPL MICROBIOL BIOT, V32, P521

[12]

PANCHAL CJ, 1979, J COLLOID INTERF SCI, V68, P296

[13] BIO-SURFACTANTS [J].

PARKINSON, M .

BIOTECHNOLOGY ADVANCES, 1985, 3 (01) :65-83

[14]

ROSENBERG E, 1986, BACTERIAL OUTER MEMB, P311

[15] EXTRACELLULAR ACCUMULATION OF TREHALOSE AND GLUCOSE BY BACTERIA GROWN ON N-ALKANES [J].

SUZUKI, T ;

TANAKA, K ;

KINOSHITA, S .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1969, 33 (02) :190-&

[16] BIO-EMULSIFIERS [J].

ZAJIC, JE ;

PANCHAL, CJ .

CRC CRITICAL REVIEWS IN MICROBIOLOGY, 1976, 5 (01) :39-66

[17] PROPERTIES AND BIODEGRADATION OF A BIO-EMULSIFIER FROM CORYNEBACTERIUM-HYDROCARBOCLASTUS [J].

ZAJIC, JE ;

GUIGNARD, H ;

GERSON, DF .

BIOTECHNOLOGY AND BIOENGINEERING, 1977, 19 (09) :1303-1320

← 1 2 →