AN OXYGEN-ELECTRODE ASSAY TO DETERMINE AUTOCLAVED-INDUCED TOXICITY IN TISSUE-CULTURE MEDIA

被引：5

作者：

HSIAO, KC

BORNMAN, CH

机构：

来源：

PHYSIOLOGIA PLANTARUM | 1991年 / 81卷 / 01期

关键词：

AUTOCLAVING; CARBOHYDRATE DEGRADATION; CYANIDE; MEDIUM TOXICITY; OXYGEN CONSUMPTION; INVITRO; SUGARS; GROWTH;

D O I：

10.1111/j.1399-3054.1991.tb01712.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

An oxygen electrode was used to assay cyanide-induced oxygen consumption of 8 sugars following autoclaving and the results correlated with the effects of the same sugars (reported by de Lange, S. Afr. J. Bot. 55, 1, 1989) on pollen germination and pollen tube growth. Of the sugars tested, fructose was the least stable, producing a degradation product that resulted in a 40-fold higher consumption of oxygen than sucrose. The use of cyanide-induced oxygen uptake makes it possible to control more carefully the conditions of preparation of sterile media by autoclaving.

引用

页码：55 / 58

页数：4

共 13 条

[1] MAILLARD BROWNING OF COMMON AMINO-ACIDS AND SUGARS [J].