PLASMODIUM-FALCIPARUM - A METHOD FOR THE AMPLIFICATION OF S-ANTIGENS AND ITS APPLICATION TO LABORATORY AND FIELD SAMPLES

被引:17
作者
KYES, S [1 ]
CRAIG, AG [1 ]
MARSH, K [1 ]
NEWBOLD, CI [1 ]
机构
[1] KEMRI, COASTAL UNIT, KILIFI, KENYA
基金
英国惠康基金;
关键词
S-ANTIGEN; MALARIA; POLYMORPHISM; STRAIN IDENTIFICATION;
D O I
10.1006/expr.1993.1108
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Polymerase chain reaction amplification of several polymorphic genes has been used to study the population biology of Plasmodium falciparum. S antigen is particularly suitable for such studies, but difficulties in the amplification of this gene have precluded its use. Here we describe a simple method for the amplification of S antigen and show why previous attempts may have been unsuccessful. Data are presented from both laboratory and field isolates. © 1993 by Academic Press, Inc.
引用
收藏
页码:473 / 483
页数:11
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  • [31] PLASMODIUM-FALCIPARUM - A SIMPLE POLYMERASE CHAIN-REACTION METHOD FOR DIFFERENTIATING STRAINS
    WOODEN, J
    GOULD, EE
    PAULL, AT
    SIBLEY, CH
    [J]. EXPERIMENTAL PARASITOLOGY, 1992, 75 (02) : 207 - 212