PLASMODIUM-FALCIPARUM - A METHOD FOR THE AMPLIFICATION OF S-ANTIGENS AND ITS APPLICATION TO LABORATORY AND FIELD SAMPLES

被引：17

作者：

KYES, S ^{[1
]}

CRAIG, AG ^{[1
]}

MARSH, K ^{[1
]}

NEWBOLD, CI ^{[1
]}

机构：

[1] KEMRI, COASTAL UNIT, KILIFI, KENYA

来源：

EXPERIMENTAL PARASITOLOGY | 1993年 / 77卷 / 04期

基金：

英国惠康基金;

关键词：

S-ANTIGEN; MALARIA; POLYMORPHISM; STRAIN IDENTIFICATION;

D O I：

10.1006/expr.1993.1108

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Polymerase chain reaction amplification of several polymorphic genes has been used to study the population biology of Plasmodium falciparum. S antigen is particularly suitable for such studies, but difficulties in the amplification of this gene have precluded its use. Here we describe a simple method for the amplification of S antigen and show why previous attempts may have been unsuccessful. Data are presented from both laboratory and field isolates. © 1993 by Academic Press, Inc.

引用

页码：473 / 483

页数：11