共 31 条
PLASMODIUM-FALCIPARUM - A METHOD FOR THE AMPLIFICATION OF S-ANTIGENS AND ITS APPLICATION TO LABORATORY AND FIELD SAMPLES
被引:17
作者:
KYES, S
[1
]
CRAIG, AG
[1
]
MARSH, K
[1
]
NEWBOLD, CI
[1
]
机构:
[1] KEMRI, COASTAL UNIT, KILIFI, KENYA
基金:
英国惠康基金;
关键词:
S-ANTIGEN;
MALARIA;
POLYMORPHISM;
STRAIN IDENTIFICATION;
D O I:
10.1006/expr.1993.1108
中图分类号:
R38 [医学寄生虫学];
Q [生物科学];
学科分类号:
07 ;
0710 ;
09 ;
100103 ;
摘要:
Polymerase chain reaction amplification of several polymorphic genes has been used to study the population biology of Plasmodium falciparum. S antigen is particularly suitable for such studies, but difficulties in the amplification of this gene have precluded its use. Here we describe a simple method for the amplification of S antigen and show why previous attempts may have been unsuccessful. Data are presented from both laboratory and field isolates. © 1993 by Academic Press, Inc.
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页码:473 / 483
页数:11
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