CLONING AND EXPRESSION OF HUMAN-BRAIN TYPE-I INOSITOL 1,4,5-TRISPHOSPHATE 5-PHOSPHATASE - HIGH-LEVELS OF MESSENGER-RNA IN CEREBELLAR PURKINJE-CELLS

被引：62

作者：

DESMEDT, F

VERJANS, B

MAILLEUX, P

ERNEUX, C

机构：

[1] FREE UNIV BRUSSELS,IRIBHN,B-1070 BRUSSELS,BELGIUM

[2] FREE UNIV BRUSSELS,BRAIN RES UNIT,B-1070 BRUSSELS,BELGIUM

来源：

FEBS LETTERS | 1994年 / 347卷 / 01期

关键词：

INOSITOL PHOSPHATE METABOLISM; SIGNAL TRANSDUCTION; CA2+; HUMAN BRAIN;

D O I：

10.1016/0014-5793(94)00509-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In brain and many other tissues, Type I inositol 1,4,5-trisphosphate (InsP(3)) 5-phosphatase is the major isozyme hydrolysing the calcium-mobilizing second messenger InsP(3). We recently reported the cloning and expression of dog thyroid InsP(3) 5-phosphatase. During the course of this cloning, screening of a human brain cDNA library allowed us to isolate a cDNA clone D1 with 91% sequence identity with the thyroid sequence. When clone D1 was expressed in Escherichia coli, the fusion protein had InsP(3) 5-phosphatase activity. M(r) estimates of the recombinant enzyme made by immunodetection, activity assay after SDS/PAGE or silver staining were consistent with the calculated molecular mass. In situ hybridization on human cerebellum sections localised the mRNA for this enzyme to the Purkinje cells.

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页码：69 / 72

页数：4

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