EXTRACELLULAR MAST-CELL GRANULES CARRY APOLIPOPROTEIN B-100-CONTAINING LIPOPROTEINS INTO PHAGOCYTES IN HUMAN ARTERIAL INTIMA - FUNCTIONAL COUPLING OF EXOCYTOSIS AND PHAGOCYTOSIS IN NEIGHBORING CELLS

被引:37
作者
KAARTINEN, M
PENTTILA, A
KOVANEN, PT
机构
[1] WIHURI RES INST,SF-00140 HELSINKI,FINLAND
[2] UNIV HELSINKI,DEPT FORENS MED,SF-00300 HELSINKI,FINLAND
关键词
ATHEROSCLEROSIS; EXOCYTOSIS; MAST CELLS; LOW-DENSITY LIPOPROTEINS; PHAGOCYTOSIS;
D O I
10.1161/01.ATV.15.11.2047
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
In experimental studies in vitro, mast cells have induced uptake of apolipoprotein B-100 (apoB-100)-containing low-density lipoproteins by macrophages, with the subsequent formation of foam cells, the hallmarks of atherosclerosis. Recently, increased numbers of activated, ie, degranulated, mast cells were found to be present in human coronary fatty streaks and atheromas. We therefore sought evidence of a connection between mast cells and foam cell formation in vivo. In electron microscopic studies of human aortic and coronary fatty streaks and atheromas, exocytosed cytoplasmic secretory granules of mast cells were detected in the vicinity of their parent cells. These exocytosed granules had bound apoB-100-containing lipoproteins, as indicated by their positive staining with MB 47, a monoclonal antibody against apoB-100. A smooth muscle cell was observed to be in the process of phagocytosing one such exocytosed granule, and in the vicinity of a degranulated mast cell a foam cell contained an ingested mast cell granule. Therefore, the micrographs show that exocytosed granules of intimal mast cells may contribute to intimal foam cell formation and suggest a role for mast cells in human atherogenesis. More generally, the findings provide evidence that phagocytosis of apoB-100-carrying particles is one mechanism by which lipoproteins enter human arterial intimal cells.
引用
收藏
页码:2047 / 2054
页数:8
相关论文
共 37 条
[31]  
STEINBERG D, 1989, NEW ENGL J MED, V320, P915
[32]   THE CELL-POPULATION OF AORTIC FATTY STREAKS IN AFRICAN-GREEN MONKEYS WITH SPECIAL REFERENCE TO GRANULOCYTIC CELLS - AN ULTRASTRUCTURAL-STUDY [J].
TRILLO, AA .
ATHEROSCLEROSIS, 1982, 43 (2-3) :259-275
[33]   COMPLEXES OF LOW-DENSITY LIPOPROTEINS AND ARTERIAL PROTEOGLYCAN AGGREGATES PROMOTE CHOLESTERYL ESTER ACCUMULATION IN MOUSE MACROPHAGES [J].
VIJAYAGOPAL, P ;
SRINIVASAN, SR ;
JONES, KM ;
RADHAKRISHNAMURTHY, B ;
BERENSON, GS .
BIOCHIMICA ET BIOPHYSICA ACTA, 1985, 837 (03) :251-261
[34]   MAST-CELL GRANULE REMNANTS CARRY LDL INTO SMOOTH-MUSCLE CELLS OF THE SYNTHETIC PHENOTYPE AND INDUCE THEIR CONVERSION INTO FOAM CELLS [J].
WANG, YF ;
LINDSTEDT, KA ;
KOVANEN, PT .
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 1995, 15 (06) :801-810
[35]  
WEISGRABER KH, 1987, J BIOL CHEM, V262, P11097
[36]  
YLAHERTTULA S, 1990, EUR HEART J SE, V11, P1086
[37]  
YOUNG SG, 1994, J LIPID RES, V35, P399