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CRYSTALLIZATION EXPERIMENTS WITH 2-ENOYL-COA HYDRATASE, USING AN AUTOMATED FAST-SCREENING CRYSTALLIZATION PROTOCOL

被引：44
作者：
ZEELEN, JP
HILTUNEN, JK
CESKA, TA
WIERENGA, RK
机构：
[1] UNIV OULU,DEPT MED BIOCHEM,SF-90220 OULU,FINLAND
[2] UNIV OULU,BIOCTR OULU,SF-90220 OULU,FINLAND
来源：
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1994年 / 50卷
关键词：
D O I：
10.1107/S0907444994001277
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A convenient method for screening crystallization conditions using an automated fast-screen protocol has been implemented and tested on an enoyl-CoA hydratase. The crystallization solutions for the initial screening and subsequent optimizations are prepared using a crystallization robot. Enoyl-CoA hydratase (E.C. 4.2.1.17), purified from rat-liver mitochondria, is one of the enzymes from the beta-oxidation pathway of fatty-acid metabolism; it catalyzes the reversible hydration of 2-trans-enoyl-CoA's to L-3-hydroxyacyl-CoA's. Different crystal forms, diffracting to 3.0 angstrom, were obtained.
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页码：443 / 447
页数：5
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