A FLEXIBLE APPROACH TO AUTOMATED PROTEIN CRYSTALLIZATION

被引：19

作者：

OLDFIELD, TJ

CESKA, TA

BRADY, RL

机构：

[1] UNIV YORK,DEPT CHEM,YORK YO1 5DD,N YORKSHIRE,ENGLAND

[2] EUROPEAN MOLEC BIOL LAB,W-6900 HEIDELBERG,GERMANY

来源：

JOURNAL OF APPLIED CRYSTALLOGRAPHY | 1991年 / 24卷

关键词：

D O I：

10.1107/S0021889891001632

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An automated system for protein crystallization trials is described. Similar to commercial systems in operation, this system is readily assembled at a far lower cost. The software runs on either a microcomputer or a VAX and is both versatile and readily modified for special requirements. The system is capable of pipetting polyethylene glycol solutions accurately. Root-mean-square deviations in the accuracy of pipetted well solutions were similar to results obtained manually. Factors influencing reproducibility are discussed.

引用

页码：255 / 260

页数：6

共 7 条

[1] A SERINE PROTEASE TRIAD FORMS THE CATALYTIC CENTER OF A TRIACYLGLYCEROL LIPASE [J].