A METHOD FOR THE HIGH-EFFICIENCY OF WATER-SOLUBLE CARBODIIMIDE-MEDIATED AMIDATION

被引：286

作者：

SEHGAL, D ^{[1
]}

VIJAY, IK ^{[1
]}

机构：

[1] UNIV MARYLAND,DEPT ANIM SCI,COLLEGE PK,MD 20742

来源：

ANALYTICAL BIOCHEMISTRY | 1994年 / 218卷 / 01期

关键词：

D O I：

10.1006/abio.1994.1144

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Water-soluble carbodiimides are widely used for carboxyl-amine conjugation. However, extremely variable and low yields, obtained under a variety of conditions, have been a serious problem in the coupling. A simple method, optimizing various parameters of the coupling reaction, in which N-hydroxysuccinimide is included to assist the 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride-catalyzed amidation reaction is described. A product yield of up to 90% is routinely achieved. (C) 1994 Academic Press, Inc.

引用

页码：87 / 91

页数：5

共 14 条

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[2]

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