ANALYSIS OF SUGARS BY SUPERCRITICAL FLUID CHROMATOGRAPHY USING POLAR PACKED-COLUMNS AND LIGHT-SCATTERING DETECTION

被引：27

作者：

HERBRETEAU, B ^{[1
]}

LAFOSSE, M ^{[1
]}

MORINALLORY, L ^{[1
]}

DREUX, M ^{[1
]}

机构：

[1] UNIV ORLEANS,UFR SCI,CHIM BIOORGAN & ANALYT LAB,BP 6759,F-45067 ORLEANS 2,FRANCE

来源：

JOURNAL OF CHROMATOGRAPHY | 1990年 / 505卷 / 01期

关键词：

D O I：

10.1016/S0021-9673(01)93088-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The application of supercritical fluid chromatography (SFC) with polar packed columns and light-scattering detection for the analysis of sugars is reported. Cyano-, diol- and nitro-bonded silicas were used with carbon dioxide-methanol mobile phases and a comparison of sugar retention was carried out. These SFC systems showed different selectivites from that found in high-performance liquid chromatography. The association of a constant flow-rate of carbon dioxide and a variable flow-rate of methanol affords the elution of mono-, di- and trisaccharides in the same analysis without baseline drift. © 1990.

引用

页码：299 / 305

页数：7

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