VISUAL READING METHOD FOR DETECTION OF BACTERIAL TANNASE

被引：64

作者：

OSAWA, R

WALSH, TP

机构：

[1] LONE PINE KOALA SANCTUARY,VET SERV & RES,BRISBANE,QLD 4069,AUSTRALIA

[2] QUEENSLAND UNIV TECHNOL,SCI LIFE SCI,CTR MOLEC BIOTECHNOL,BRISBANE,QLD 4001,AUSTRALIA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1993年 / 59卷 / 04期

关键词：

D O I：

10.1128/AEM.59.4.1251-1252.1993

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Tannase activity of bacteria capable of degrading tannin-protein complexes was determined by a newly developed visual reading method. The method is based on two phenomena: (i) the ability of tannase to hydrolyze methyl gallate to release free gallic acid and (ii) the green to brown coloration of gallic acid after prolonged exposure to oxygen in an alkaline condition. The method has been successfully used to detect the presence of tannase in the cultures of bacteria capable of degrading tannin-protein complexes.

引用

页码：1251 / 1252

页数：2

共 10 条

[1] PURIFICATION AND SOME PROPERTIES OF YEAST TANNASE [J].