GENETIC ORGANIZATION OF THE KPNI RESTRICTION MODIFICATION SYSTEM

被引：14

作者：

CHATTERJEE, DK

HAMMOND, AW

BLAKESLEY, RW

ADAMS, SM

GERARD, GF

机构：

[1] Life Technologies, Inc., Gaithersburg, MD 20877

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 23期

关键词：

D O I：

10.1093/nar/19.23.6505

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The KpnI restriction-modification (KpnI RM) system was previously cloned and expressed in E. coli. The nucleotide sequences of the KpnI endonuclease (R.KpnI) and methylase (M. KpnI) genes have now been determined. The sequence of the amino acid residues predicted from the endonuclease gene DNA sequence and the sequence of the first 12 NH2-terminal amino acids determined from the purified endonuclease protein were identical. The kpnIR gene specifies a protein of 218 amino acids (MW: 25, 115), while the kpnIM gene codes for a protein of 417 amino acids (MW: 47,582). The two genes transcribe divergently with a intergenenic region of 167 nucleotides containing the putative promoter regions for both genes. No protein sequence similarity was detected between R.KpnI and M.KpnI. Comparison of the amino acid sequence of M.KpnI with sequences of various methylases revealed a significant homology to N6-adenine methylases, a partial homology to N4-cytosine methylases, and no homology to C5-methylases.

引用

页码：6505 / 6509

页数：5

共 39 条

[21] PURIFICATION, CLONING AND SEQUENCE-ANALYSIS OF RSRI DNA METHYLTRANSFERASE - LACK OF HOMOLOGY BETWEEN 2 ENZYMES, RSRI AND ECORI, THAT METHYLATE THE SAME NUCLEOTIDE IN IDENTICAL RECOGNITION SEQUENCES [J].